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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
20/07/2022 |
Data da última atualização: |
20/07/2022 |
Autoria: |
MATIELLO, J. B.; SANTINATO, R.; CAMARGO, A. P. de; ALMEIDA, S. R.; FERNANDES, D. R.; D'ANTONIO, A. M.; GARCIA, A. W. R.; CORTEZ, G.; VIANA, A. S.; MIGUEL, A. E.; CARVALHO, S. P. |
Título: |
A moderna cafeicultura nos cerrados: instrucoes tecnicas sobre a cultura de cafe no Brasil. |
Ano de publicação: |
1987 |
Fonte/Imprenta: |
Rio de Janeiro: IBC, 1987 |
Páginas: |
148p. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Coffee; Controle; Cultivation; Cultivo; Cultural methods; Spacing. |
Thesagro: |
Brazil; Café; Cerrado; Clima; climate; Coffea Arábica; disease control; Doença de Planta; Espaçamento; Nutrição Vegetal; pest control; plant nutrition; Praga de Planta; Pratica Cultural; soil; Solo; Variedade; varieties. |
Categoria do assunto: |
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Marc: |
LEADER 01315nam a2200529 a 4500 001 1023979 005 2022-07-20 008 1987 bl uuuu 00u1 u #d 100 1 $aMATIELLO, J. B. 245 $aA moderna cafeicultura nos cerrados$binstrucoes tecnicas sobre a cultura de cafe no Brasil. 260 $aRio de Janeiro: IBC$c1987 300 $a148p. 650 $aBrazil 650 $aCafé 650 $aCerrado 650 $aClima 650 $aclimate 650 $aCoffea Arábica 650 $adisease control 650 $aDoença de Planta 650 $aEspaçamento 650 $aNutrição Vegetal 650 $apest control 650 $aplant nutrition 650 $aPraga de Planta 650 $aPratica Cultural 650 $asoil 650 $aSolo 650 $aVariedade 650 $avarieties 653 $aBrasil 653 $aCoffee 653 $aControle 653 $aCultivation 653 $aCultivo 653 $aCultural methods 653 $aSpacing 700 1 $aSANTINATO, R. 700 1 $aCAMARGO, A. P. de 700 1 $aALMEIDA, S. R. 700 1 $aFERNANDES, D. R. 700 1 $aD'ANTONIO, A. M. 700 1 $aGARCIA, A. W. R. 700 1 $aCORTEZ, G. 700 1 $aVIANA, A. S. 700 1 $aMIGUEL, A. E. 700 1 $aCARVALHO, S. P.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
30/12/2014 |
Data da última atualização: |
01/09/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
COSTA, H. B.; FERNANDES, P. M. B.; ROMÃO, W.; VENTURA, J. A. |
Afiliação: |
Helber B. Costa; Patricia M.B. Fernandes; Wanderson Romão; Jose Aires Ventura, Incaper. |
Título: |
A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Industrial Crops and Products, Vol. 59, p. 163-168, august 2014. |
Páginas: |
6 p. |
Idioma: |
Inglês |
Conteúdo: |
Bioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. |
Thesaurus NAL: |
Bromelain; Enzyme; Forbromelain; Pineapple; Purification. |
Categoria do assunto: |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/419/1/New-Procedure-Chromatography-to-Purify-Bromelain-2014.pdf
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Marc: |
LEADER 02130naa a2200229 a 4500 001 1004754 005 2015-09-01 008 2014 bl uuuu u00u1 u #d 100 1 $aCOSTA, H. B. 245 $aA new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies.$h[electronic resource] 260 $c2014 300 $a6 p. 520 $aBioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. 650 $aBromelain 650 $aEnzyme 650 $aForbromelain 650 $aPineapple 650 $aPurification 700 1 $aFERNANDES, P. M. B. 700 1 $aROMÃO, W. 700 1 $aVENTURA, J. A. 773 $tIndustrial Crops and Products, Vol. 59, p. 163-168, august 2014.
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